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刘奋勇教授
2014-03-25 10:40   发布范围:公开

刘奋勇教授,国家“千人计划”特聘教授,第四军医大学讲座教授,美国加州大学伯克利分校终身教授。1986年于中国科技大学获学士学位。1987-1992年在芝加哥大学师从Dr. Bernard Roizman (疱疹病毒专家、美国科学院院士),开展单纯疱疹病毒1型蛋白酶的研究,并于1989、1992年获硕士、博士学位。1992-1995年在耶鲁大学跟随Dr. Sidney Altman (RNA专家、诺贝尔化学奖得主)进行博士后研究,从事利用核酶抑制疱疹病毒基因表达的研究。1996年被加州大学伯克利分校聘为助理教授,2001年晋升为副教授,2005年晋升为正教授(tenured full professor),2006年任加州大学伯克利分校比较生物学项目主席,2008年任加州大学伯克利分校传染病系副主任。2008年作为国家首批“千人计划”入选者到武汉大学任教授、博士生导师。一直从事HSV-1、HCMV、KSHV等疱疹病毒的研究。以通讯作者身份在PNAS、J Virol、JBC、Nucleic Acids Res等有影响的国际期刊发表学术论文超过100篇,获准美国发明专利7项。近年的研究聚焦在通过阐明HCMV基因在致病和复制中的功能来理解巨细胞病毒感染的生物学,同时发展新的基于RNA技术的生物治疗剂,阐明其抗病毒效率及作用机制。首次对HCMV基因组162个基因进行的系统功能分析被视为疱疹病毒研究领域里程碑式的贡献,鉴定出全部病毒复制必需基因并首次发现病毒节制因子,揭示了一种崭新的病毒调控机制。针对HCMV蛋白酶等靶标,研制出基于Rnase P核酶、外部引导序列(EGS)和RNA适配子等多种新型技术的抗病毒治疗剂。

代表性论文:

1.Liu, F., and Roizman, B. (1991). The herpes simplex virus 1 gene encoding a protease also contains within its coding domain the gene encoding the more abundant substrate. J. Virol. 65: 5149-5156.

2.Liu, F., and Roizman, B. (1992).Differentiation of multiple domains in the herpes simplex virus 1 protease encoded by the UL26 gene.Proc. Natl. Acad. Sci. USA. 89: 2076-2080.

3.Liu, F., and Roizman, B. (1993). Characterization of the protease and of other products of the amino terminus proximal cleavage of the herpes simplex virus 1 UL26 protein.J. Virol. 67, 1300-1309.

4.Liu, F., and Altman, S. (1994).Differential evolution of substrates for an RNA enzyme in the presence and absence of its protein cofactor. Cell, 77, 1093-1100.

5.Liu, F., and Altman, S. (1995). Inhibition of viral gene expression by the catalytic RNA subunit of RNase P for Escherichia coli.Genes & Development, 9, 471-480.

6.Kim, J., Kilani, A., Zhan, X., Altman, S., and Liu, F. (1997). The protein cofactor allows the sequence of an RNase P ribozyme to diversify by maintaining the catalytically active structure of the enzyme. RNA, 3, 613-623.

7.Kawa, D., Wang, J., Yuan, Y., and Liu, F. (1998). Inhibition of viral gene expression by human ribonuclease P.RNA. 4: 1397-1406.

8.Chen, D., Jiang, H., Lee, M., Liu, F., and Zhou, H. (1999).Three-dimensional visualization of tegument/capsid interactions in intact human cytomegalovirus.Virology, 260, 10-16.

9.Kilani, A. F. andLiu, F. (1999). UV-crosslink mapping of the substrate-binding site of RNase P ribozyme to a target mRNA sequence. RNA, 5, 1235-1247.

10.Trang, P., Hsu, A., and Liu, F. (1999). Nuclease footprint analyses of the interactions between RNase P ribozyme and a model mRNA substrate.Nucleic Acids Res. 27, 4590-4597.

11.Zhan, X., Abenes, G., Lee, M., VonReis, I. , Kittikarookoon, C., Ross-Macdonald, P., Snyder, M., andLiu, F. (2000). Mutagenesis of murine cytomegalovirus using a Tn3-based transposon.Virology, 266, 264-274.

12.Kilani, A. F., Trang, P., Jo, S. Hsu, A., Kim, J., Nepomuceno, E., and Liu, F. (2000).RNase P ribozymes selected in vitro to cleave a viral mRNA effectively inhibit its expression in cell culture.J. Biol. Chem.,275, 10611-10622.

13.Wang, J., Jiang, H., and Liu, F. (2000). In vitro selection of novel RNA ligands that bind human cytomegalovirus and block viral infection.RNA, 6, 571-583.

14.Trang, P., Lee, M., Nepomuceno, E., Kim, J., Zhu, H., and Liu, F. (2000).Effective inhibition of humancytomegalovirus gene expression and replication by a RNase P ribozyme. Proc. Natl. Acad. Sci. USA, 97, 5812-5817.

15.Liu, F., Wang, J., and Trang, P. (2000). In vitro selection of substrates by RNase P ribozymes.Methods in Enzymology, Vol 318, 238-250.

16.Zhan, X., Lee, M., Xiao, J., and Liu, F. (2000).Construction and characterization of murine cytomegalovirus mutants that contain a transposon insertion at open reading frames m09 and M83.J. Virol. 74, 7411-7421.

17.Hsu, A. W., Kilani, A. F., Liou, K., Lee, J., and Liu, F. (2000).Differential effects of the protein cofactor on the interactions between an RNase ribozyme and its target mRNA substrate. Nucleic Acids Res.28, 3105-3116.

18.Trang, P., Kilani, A. F., Kim, J., and Liu, F. (2000). A ribozyme derived from the catalytic subunit of RNase P from Escherichia coli is highly effective in inhibiting replication of herpes simplex virus 1. J. Mol. Biol.,301, 817-826.

19.Xiao, J., Tuong, T., Zhan, X., Haghjoo, E., and Liu, F. (2000) In vitro and in vivo characterization of a murine cytomegalovirus with a transposon insertional mutation at open reading frame M43.J. Virol., 74, 9488-9497.

20.Lee, M., Xiao, J., Haghjoo, E., Tong, T., Abenes, G., Zhan, X., Dunn, W., and Liu, F. (2000) Murine cytomegalovirus containing a mutation at open reading frame M37 is severely attenuated in growth and virulence in vivo.J. Virol. 74, 11099-11107.

著作:

Liu, F., and Altman, S. (2010) Ribonuclease P. Springer, New York.

发明专利:

申报8件美国专利,7件已经获得授权。

(1)Roizman, B. and Liu, F. (1995).Methods and compositions for the preparation and use of a herpes protease. U.S. Patent No. 5,478,727. (December 26, 1995), U.S. Patent and Trademark Office.

(2)Yuan, Y. Guerrier-Takada, C. Altman, S. and Liu, F. (1997).Targeted cleavage of RNA using eukaryotic ribonuclease P and external guide sequence. U.S. Patent No. 5,624,824 (April 29, 1997).U.S. Patent and Trademark Office.

(3)Yuan, Y. Guerrier-Takada, C. Altman, S. and Liu, F. (1998).Targeted cleavage of RNA using eukaryotic ribonuclease P and external guide sequence. U.S. Patent No. 5,728,521 (March 17, 1998).U.S. Patent and Trademark Office.

(4)Yuan, Y. Guerrier-Takada, C. Altman, S. and Liu, F. (1999).Targeted cleavage of RNA using ribonuclease P and external guide sequence. U.S. Patent No. 5,869,248 (February 9, 1999).U.S. Patent and Trademark Office.

(5)Roizman, B. and Liu, F. (2002).Methods and compositions for the preparation and use of a herpes protease. U.S. Patent No. 6,410,704. (July 25, 2002), U.S. Patent and Trademark Office.

(6)Liu, F., Wang, J., and Jiang, H. (2005). A method to generate nucleic acid ligands that bind herpesviruses and inhibit their infection.U.S. Patent No. 6,849,610 (February 1, 2005).U.S. Patent and Trademark Office.

(7)Liu, F., Dunn, W., and Chou, C. (2008). Cytomegalovirus gene function and methods for developing antivirals, anti-CMV vaccines, and CMV-based vectors.U.S. Patent No. 7,407,744 (August 5, 2008).U.S. Patent and Trademark Office (Submitted on July 25, 2003).

(8)Liu, F. Gong, H., Bai, Y., and Lu, S. (2013).Oral Delivery of Nucleic Acid-Based Gene Interfering Agents by Salmonella.U.S. Patent and Trademark Office (Submitted on January 30, 2013).

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